Lehrinhalte
The course is an integrated course consisting of a series of lectures and a practical part.
Topics covered in the lecture series:
1. Overview of environmentally relevant microbial processes (nitrogen cycle, organohalide respiration, transformation of xenobiotics, etc)
2. Molecular fingerprinting techniques that are commonly used in research and practice of environmental engineering
3. Proteomics approaches in characterization of environmentally relevant biotransformation processes (e.g., for degradation of pesticides, artificial sweetener, antibiotics, etc)
4. Reactor & sensor technology and its application
The Practical course will be a four day program that covers two parts:
1. Construction and start-up of a continuous lab-scale bioreactor using Raspberry Pi microcomputer, a syringe pump, and necessary sensors; simple python programming excersise
2. Microbial community analysis of samples of interests (can be known microbial samples or unknown samples with defined sources, non-pathogenic) using DGGE of 16S rRNA gene amplicons. DNA extraction, PCR, DGGE, gel band excision, and sequencing will be performed.
